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KMID : 0377619690160010045
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Korean Jungang Medical Journal
1969 Volume.16 No. 1 p.45 ~ p.54
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Immunologic Study of Human Chorionic Gonadotropin in Serum of Pregnant Women
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Abstract
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For the immunologic pregnancy test and determination of H. C. G. in pregnant serum, basic experiments and semi-quantitative assay by means of hemagglutination inhibition technique has been performed, and the obtained results are as following.
1. Relatively pure H. C. G. (5,427 IU/mg) was used as antigen for immunization of rabbits and high titer of antiserum was obtained through the effects of additional booster injections and complete Freund´s adjuvant.
2. The sensitivity of hemagglutination inhibition reaction was very high. The minimum amount of H. C. G. detected was 0. 3 IU/ml.
3. Double diffusion method of Ouchterlony used in this study for the analysis of H. C. G. showed only one clear precipitin line between antigen and antiserum, indicating that the used H. C. G. is relatively pure.
4. The purity of antigen was proved by absorption of antiserum with urine or serum extract from non-pregnant women tested in hemagglutination reaction andhemagglutination inhibition reaction.
5. Incidence of false reaction was very high when the serum was not treated by acetone precipitation in both pregnant and non-pregnant women.
6. Recovery rate of H. C. G. with the acetone precipitiation method was about 95 per cent.
7. When the sensitivity of hemagglutination inhibition reaction was so adjusted that more than 1.2-2.4 IU/ml. of H. C. G. could be detectable in serum of 1 : 277 and 1 : 4 dilution, accuracy of pregnancy test on sera from 65 normal pregnant women of first trimester was 98. 5 per cent.
8. A high accuracy of hundred, per cent was obtained in 20 nonpregnant sera, without even a single- case of false positive.
9. The semi-quantitative assay of H. C. G. in the serum f nom 207 normal pregnant women at various gestational poriod revealed that H. C. G. could be detected after 37 days from L. M.P., thereafter sharply increased and reached to the peak in 60-80 days, giving an average of 108 IU/ml, than declined to reach to 22. 4-37.6 IU/ml, average level in latex half of pregnancy.
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